ang ii enzyme linked immunosorbent assay elisa kit  (Proteintech)

Journal: International Journal of Molecular Sciences

Article Title: Piper sarmentosum Roxb. Inhibits Angiotensin-Converting Enzyme Activity in Phorbol 12-Myristate-13-Acetate-Induced Endothelial Cells

doi: 10.3390/ijms25052806

Figure Lengend Snippet: Determination of the optimum concentrations of PMA, AEPS, and captopril for HUVEC treatments based on ACE activity. ( A ) ACE activity in HUVECs induced with 100–300 nM PMA, ( B ) ACE activity in PMA-induced HUVECs upon treatment with different concentrations of AEPS (100–300 µg/mL), and ( C ) ACE activity in PMA-induced HUVECs upon treatment with different concentrations of captopril (0.02–0.06 µM). Values are expressed as mean ± SEM, n = 6. * p < 0.05, ** p < 0.01 compared with the untreated control, # p < 0.05, ## p < 0.01, ### p < 0.001 compared with the PMA group. ACE, angiotensin-converting enzyme; AEPS, aqueous extract of Piper sarmentosum leaves; PMA, phorbol 12-myristate-13-acetate.

Article Snippet: The ACE and Ang II protein levels in HUVECs were determined using Human Ang II and ACE enzyme-linked immunosorbent assay (ELISA) kits (FineTest, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Activity Assay

Journal: International Journal of Molecular Sciences

Article Title: Piper sarmentosum Roxb. Inhibits Angiotensin-Converting Enzyme Activity in Phorbol 12-Myristate-13-Acetate-Induced Endothelial Cells

doi: 10.3390/ijms25052806

Figure Lengend Snippet: Effects of AEPS treatment on ( A ) ACE mRNA expression and ( B ) ACE protein levels in HUVECs induced with PMA. Values are expressed as mean ± SEM, n = 6. *** p < 0.001 compared with control, ## p < 0.01, ### p < 0.001 compared with PMA group. ACE, angiotensin-converting enzyme; AEPS, aqueous extract of Piper sarmentosum leaves; PMA, phorbol 12-myristate-13-acetate.

Article Snippet: The ACE and Ang II protein levels in HUVECs were determined using Human Ang II and ACE enzyme-linked immunosorbent assay (ELISA) kits (FineTest, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Piper sarmentosum Roxb. Inhibits Angiotensin-Converting Enzyme Activity in Phorbol 12-Myristate-13-Acetate-Induced Endothelial Cells

doi: 10.3390/ijms25052806

Figure Lengend Snippet: Effects of AEPS treatment on ACE activity in PMA-induced HUVECs. Values are expressed as mean ± SEM, n = 6. ** p < 0.01 compared with control. ## p < 0.01 compared with PMA group. ACE, angiotensin-converting enzyme; AEPS, aqueous extract of Piper sarmentosum leaves; PMA, phorbol 12-myristate-13-acetate.

Article Snippet: The ACE and Ang II protein levels in HUVECs were determined using Human Ang II and ACE enzyme-linked immunosorbent assay (ELISA) kits (FineTest, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Activity Assay

Journal: International Journal of Molecular Sciences

Article Title: Piper sarmentosum Roxb. Inhibits Angiotensin-Converting Enzyme Activity in Phorbol 12-Myristate-13-Acetate-Induced Endothelial Cells

doi: 10.3390/ijms25052806

Figure Lengend Snippet: Effects of AEPS treatment on angiotensin II protein levels in PMA-induced HUVEC. Values are expressed as mean ± SEM, n = 6. *** p < 0.001 compared with control. # p < 0.05, ### p < 0.001 compared with PMA group. ACE, angiotensin-converting enzyme; AEPS, aqueous extract of Piper sarmentosum leaves; PMA, phorbol 12-myristate-13-acetate.

Article Snippet: The ACE and Ang II protein levels in HUVECs were determined using Human Ang II and ACE enzyme-linked immunosorbent assay (ELISA) kits (FineTest, Wuhan, China) according to the manufacturer’s instructions.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Piper sarmentosum Roxb. Inhibits Angiotensin-Converting Enzyme Activity in Phorbol 12-Myristate-13-Acetate-Induced Endothelial Cells

doi: 10.3390/ijms25052806

Figure Lengend Snippet: Effect of AEPS on ( A ) AT1R and ( B ) AT2R mRNA expressions in PMA-induced HUVEC. Values are expressed as mean ± SEM, n = 6. * p < 0.05, ** p < 0.01 compared with control. ## p < 0.01, ### p < 0.001 compared with PMA group. ACE, angiotensin-converting enzyme; AEPS, aqueous extract of Piper sarmentosum leaves; AT1R, angiotensin II type 1 receptor; AT2R, angiotensin II type 2 receptor; PMA, phorbol 12-myristate-13-acetate.

Article Snippet: The ACE and Ang II protein levels in HUVECs were determined using Human Ang II and ACE enzyme-linked immunosorbent assay (ELISA) kits (FineTest, Wuhan, China) according to the manufacturer’s instructions.

Techniques:

Journal: Journal of advanced research

Article Title: Vitamin D/VDR regulates peripheral energy homeostasis via central renin-angiotensin system.

doi: 10.1016/j.jare.2021.01.011

Figure Lengend Snippet: Fig. 5. Intracerebroventricular administration of renin significantly enhanced the ablation of VD signaling system-stimulated energy expenditure. (A) Representative images of immunofluorescence staining of renin of frozen hypothalamus sections (200). (B) Serum ANG II levels were determined by enzyme linked immunosorbent assay (ELISA); (C) VDR protein in hypothalamus; (D) Body weight; (E) Oxygen consumption; (F) Respiratory exchange ratio (RER); (G) Average basal rectal temperature; (H) Blood pressure; (I) Heart rate. WTD group was treated by supplying cholecalciferol cholesterol emulsion (CCE) in the drinking water (CCE: water = 10 ll: 100 ml) for 2 weeks. WT: wild-type mice group; KO: knockout mice group; VD: vitamin D; VDR: vitamin D receptor; VO2: oxygen consumption; ANG: angiotensin. Values are the mean ± s.e.m (n = 5–7 rats in each group), *P < 0.05; **P < 0.01.

Article Snippet: Enzyme linked immunosorbent assay (ELISA) Serum ANG II levels were determined using a mouse angiotensin II enzyme linked immunosorbent assay (ELISA) kit (CSBE04495m)(CUSABIO BIOTECH CO.,LTD, USA) according to the manufacturer’s instructions.

Techniques: Staining, Enzyme-linked Immunosorbent Assay, Emulsion, Knock-Out

Journal: Journal of advanced research

Article Title: Vitamin D/VDR regulates peripheral energy homeostasis via central renin-angiotensin system.

doi: 10.1016/j.jare.2021.01.011

Figure Lengend Snippet: Fig. 6. VD system in the central nervous system regulates energy homeostasis via RAS pathways. (A) Summary data of relative protein levels; (B) showed CRH protein expressions in hypothalamus using western blot analysis. (C) The relative protein levels are shown as relative to GAPDH protein levels determined using Graphpad Prism 5. (D) Serum ANG Ⅱlevels were determined by enzyme linked immunosorbent assay (ELISA); (E) Functional interaction between VDR and renin promoter was evaluated by Chromatin Immunoprecipitation (CHIP) assay; (F) Representative images of immunofluorescence staining of CRH of frozen hypothalamus sections (200). WTD group was treated by supplying cholecalciferol cholesterol emulsion (CCE) in the drinking water (CCE: water = 10 ll: 100 ml) for 2 weeks. WT: wild-type mice group; KO: knockout mice group; VD: vitamin D; VDR: vitamin D receptor; RAS: renin-angiotensin system (RAS); ANG II: angiotensin II; CRH: corticotropin-releasing hormone. (+): intracerebroven- tricular (ICV) injection of 1 ll renin (0.2 10-3 lg/ll), (): ICV injection of 1 ll PBS. Values are the mean ± s.e.m (n = 5–7 rats in each group), *P < 0.05; **P < 0.01.

Article Snippet: Enzyme linked immunosorbent assay (ELISA) Serum ANG II levels were determined using a mouse angiotensin II enzyme linked immunosorbent assay (ELISA) kit (CSBE04495m)(CUSABIO BIOTECH CO.,LTD, USA) according to the manufacturer’s instructions.

Techniques: Western Blot, Enzyme-linked Immunosorbent Assay, Functional Assay, Chromatin Immunoprecipitation, Staining, Emulsion, Knock-Out, Injection

Journal: Journal of advanced research

Article Title: Vitamin D/VDR regulates peripheral energy homeostasis via central renin-angiotensin system.

doi: 10.1016/j.jare.2021.01.011

Figure Lengend Snippet: Fig. 7. The VD signaling system regulates energy homeostasis via altered sympathetic nerve activity. (A) Representative electrograms from sympathetic nerves; (B) Adrb3 mRNA in WAT. (C) Adrb3 mRNA in BAT; (D) showed UCP1 and PGC1a proteins in BAT; (E) demonstrated PPARc proteins in WAT; (F) The relative protein levels are shown as relative to GAPDH protein levels determined using Graphpad Prism 5. (G) Oxygen consumption; (H) Respiratory exchange ratio (RER); (I) Summary data of relative protein levels were determined by western blot. WT group was the control group of wild-type mice. WTD group was treated by supplying cholecalciferol cholesterol emulsion (CCE) in the drinking water (CCE: water = 10 ll: 100 ml) for 2 weeks. KO group is the Cyp27b1/ mice. WTA group, WTDA group, KOA group were treated by Aliskiren respectively (0.2 mg/ml, 1 ll/d) (Renin inhibitor). VD: vitamin D; WAT: white adipose tissue; BAT: brown adipose tissue; UCP1: uncoupling protein 1; PPARc: peroxisome proliferators- activated receptor c; VO2: oxygen consumption; ANG II: angiotensin II; CRH: corticotropin-releasing hormone; ADRB3:Beta 3 adrenergic receptor; PGC1a: peroxlsome proliferator-activated receptor-c coactlvator-1a; AT1: angiotensin II type I receptor; AT2: angiotensin II type II receptor. (+): intracerebroventricular (ICV) injection of alisiken (0.2 mg/ml, 1 ll/d) for seven days, (): ICV injection of 1 ll PBS; Values are the mean ± s.e.m (n = 5–7 rats in each group), *P < 0.05; **P < 0.01; ***P < 0.001.

Article Snippet: Enzyme linked immunosorbent assay (ELISA) Serum ANG II levels were determined using a mouse angiotensin II enzyme linked immunosorbent assay (ELISA) kit (CSBE04495m)(CUSABIO BIOTECH CO.,LTD, USA) according to the manufacturer’s instructions.

Techniques: Activity Assay, Western Blot, Control, Emulsion, Injection

Journal: Critical Care Medicine

Article Title: Efficacy of Serum Angiotensin II Levels in Prognosis of Patients With Coronavirus Disease 2019

doi: 10.1097/ccm.0000000000004967

Figure Lengend Snippet: Figure 1. A, The median of the serum angiotensin (Ang) II levels in the patients with coronavirus disease 2019 (COVID-19) was 433.61 pg/mL. The median value of the serum Ang II levels of healthy controls was 774.75 pg/mL. B, The median of the first Ang II levels was 433.61 pg/mL, the second was 407.92 pg/mL, and the third was 446.09 pg/mL.

Article Snippet: The human angiotensin II enzyme-linked immunosorbent assay (ELISA) kit (Elabscience Biotechnology Catalog No: E-EL-H0326, Houston, TX) was used for the determination of serum angiotensin II levels.

Techniques:

Journal: Critical Care Medicine

Article Title: Efficacy of Serum Angiotensin II Levels in Prognosis of Patients With Coronavirus Disease 2019

doi: 10.1097/ccm.0000000000004967

Figure Lengend Snippet: Figure 2. A, In the first blood samples that were collected, the median of the Ang II levels in the patients with acute respiratory distress syndrome (ARDS) was 379.44 pg/mL (IQR, 15.00–1,455.98 pg/mL), whereas the median of the angiotensin (Ang) II levels in the patients without ARDS was 490.68 pg/mL (interquartile range [IQR], 21.65–2,955.67 pg/mL). In the second blood samples, the median of the Ang II levels in the patients with ARDS was 319.28 pg/mL (IQR, 44.06–1,336.68 pg/mL), whereas the median of Ang II levels in the patients without ARDS was 508.98 pg/mL (IQR, 10–2,062.609 pg/mL). In the third blood samples, the median of the Ang II levels in the patients with ARDS was 400.09 pg/mL (IQR, 14.61–1,049.52 pg/mL), whereas the median of the Ang II levels in the patients without ARDS was 500.39 pg/mL (IQR, 26.36–5,320.39 pg/mL). B, The median values of the serum Ang II levels in the first, second, and third blood samples of the patients admitted to the ICU were 381.68 pg/mL (IQR, 15.00–1,455.98 pg/mL), 298.23 pg/mL (IQR, 37.13–1,336.68 pg/mL), and 401.68 pg/mL (IQR, 14.61–1,049.52 pg/mL), respectively. In patients who did not require admission to the ICU, the serum Ang II levels were 495.19 pg/mL (IQR, 21.65–2,955.67 pg/mL), 531.61 pg/mL (IQR, 10.00–2,062.60), and 485.73 pg/mL (IQR, 14.61–1,049.52 pg/mL), respectively. C, In the first collected blood samples, the median of the Ang II levels in the nonsurvivors was 400.44 pg/mL (IQR, 15.00–1,455.98 pg/mL), whereas the median of the Ang II levels in the survivors was 454.27 pg/mL (IQR: 21.65–2,955.67 pg/mL) pg/mL. In the second blood samples, the median of the Ang II levels in the nonsurvivors was 360.91 pg/mL (IQR, 50–1,336 pg/mL), whereas the median of Ang II levels in the survivors was 484.4 pg/mL (IQR, 10.00–1,062.60 pg/mL). In the third blood samples, the median of the Ang II levels in the nonsurvivors was 401.68 pg/mL (IQR, 14.61–1,049.52 pg/ mL), whereas the median of Ang II levels in the survivors was 450.72 pg/mL (IQR, 26.36–5,320.79 pg/mL).

Article Snippet: The human angiotensin II enzyme-linked immunosorbent assay (ELISA) kit (Elabscience Biotechnology Catalog No: E-EL-H0326, Houston, TX) was used for the determination of serum angiotensin II levels.

Techniques: